diff --git a/doc/classes/ProjectSettings.xml b/doc/classes/ProjectSettings.xml
index 90a11a10323..6f786c5cf11 100644
--- a/doc/classes/ProjectSettings.xml
+++ b/doc/classes/ProjectSettings.xml
@@ -1967,6 +1967,9 @@
 		<member name="navigation/3d/default_link_connection_radius" type="float" setter="" getter="" default="1.0">
 			Default link connection radius for 3D navigation maps. See [method NavigationServer3D.map_set_link_connection_radius].
 		</member>
+		<member name="navigation/3d/default_up" type="Vector3" setter="" getter="" default="Vector3(0, 1, 0)">
+			Default up orientation for 3D navigation maps. See [method NavigationServer3D.map_set_up].
+		</member>
 		<member name="navigation/3d/use_edge_connections" type="bool" setter="" getter="" default="true">
 			If enabled 3D navigation regions will use edge connections to connect with other navigation regions within proximity of the navigation map edge connection margin. This setting only affects World3D default navigation maps.
 		</member>
diff --git a/scene/resources/world_3d.cpp b/scene/resources/world_3d.cpp
index 297d219caf3..b8646c53871 100644
--- a/scene/resources/world_3d.cpp
+++ b/scene/resources/world_3d.cpp
@@ -68,6 +68,7 @@ RID World3D::get_navigation_map() const {
 		NavigationServer3D::get_singleton()->map_set_active(navigation_map, true);
 		NavigationServer3D::get_singleton()->map_set_cell_size(navigation_map, GLOBAL_GET("navigation/3d/default_cell_size"));
 		NavigationServer3D::get_singleton()->map_set_cell_height(navigation_map, GLOBAL_GET("navigation/3d/default_cell_height"));
+		NavigationServer3D::get_singleton()->map_set_up(navigation_map, GLOBAL_GET("navigation/3d/default_up"));
 		NavigationServer3D::get_singleton()->map_set_use_edge_connections(navigation_map, GLOBAL_GET("navigation/3d/use_edge_connections"));
 		NavigationServer3D::get_singleton()->map_set_edge_connection_margin(navigation_map, GLOBAL_GET("navigation/3d/default_edge_connection_margin"));
 		NavigationServer3D::get_singleton()->map_set_link_connection_radius(navigation_map, GLOBAL_GET("navigation/3d/default_link_connection_radius"));
diff --git a/servers/navigation_server_3d.cpp b/servers/navigation_server_3d.cpp
index 4da479639d3..89d45b19299 100644
--- a/servers/navigation_server_3d.cpp
+++ b/servers/navigation_server_3d.cpp
@@ -192,6 +192,7 @@ NavigationServer3D::NavigationServer3D() {
 
 	GLOBAL_DEF_BASIC("navigation/3d/default_cell_size", 0.25);
 	GLOBAL_DEF_BASIC("navigation/3d/default_cell_height", 0.25);
+	GLOBAL_DEF("navigation/3d/default_up", Vector3(0, 1, 0));
 	GLOBAL_DEF("navigation/3d/use_edge_connections", true);
 	GLOBAL_DEF_BASIC("navigation/3d/default_edge_connection_margin", 0.25);
 	GLOBAL_DEF_BASIC("navigation/3d/default_link_connection_radius", 1.0);